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Next Generation Sequencing with the Illumina GAIIx and HiSeq 2000 systems

As a leading provider of genomic research services in Europe, Source BioScience LifeSciences is pleased to offer next generation sequencing using the Illumina GAIIx and HiSeq 2000. With two GAIIx, one HiSeq 2000  and two cBot cluster generation systems, we offer an experienced service with fast turnaround time, competitive quotes and provide researchers access to all recently launched applications.

 

Sequencing Technology

The Illumina sequencing system is a groundbreaking platform for genetic analysis and functional genomics. It dramatically improves speed and reduces costs, transforming the way many experiments are devised and carried out. Both the GAIIx and the HiSeq 2000 leverage on the proprietary reversible terminators and Clonal Single Molecule Array technology. The GAIIx system generates up to five hundred million reads per run and the HiSeq 2000 delivers up to two billion reads per run, generating the industry's highest sequencing output and fastest data generation rate.

 

Services Available

• Genome Sequencing
  • Single and paired-end reads allow the discovery and confirmation of mutations, chromosomal rearrangements, de novo sequencing and assembly

 

• Transcriptome Sequencing
  • Generates genome-wide expression profiles through sequencing and not hybridization
  • RNA sequencing (RNA seq), small RNA sequencing, DEEP CAGE analysis.

 

• ChIP sequencing (ChIP seq)
  • To identify and quantify in vivo protein-DNA interactions on a genome-wide scale

 

• Bioinformatics solutions
  • Our Bioinformatics scientists provide a complete analysis package that can be customised to your specific research needs

 

• Sample indexing
  • DNA samples are tagged with a unique identifier during sample preparation and up to 12 samples can be pooled into a single lane to be sequenced together. This can save time and can be a cost effective solution for sequencing

 

• Targeted sequencing
  • Specific targeted genomic regions (either contiguous or dispersed) are enriched for subsequent deep sequencing. The ability to analyse only targeted regions rather than the entire complex genome allows for effective and systematic in-depth study of genetic variation, enabling researchers to work with larger number of samples. We are pleased to offer target-specific sequence selection through Agilent SureSelect™.

 

• Paired end sequencing
  • Allows selection of a length of insert and sequencing both ends of the insert, allowing for a highly precise alignment of reads

 

Quality Assurance

We are a CSPro certified provider of Illumina sequencing. Our laboratory is also GLP, GCP and CPA accredited.